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KMID : 0545120090190101244
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 10 p.1244 ~ p.1249
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Phoma herbarum as a New Gibberellin-Producing and Plant Growth-Promoting Fungus
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Hamayun Muhammad
Sumera Afzal Khan
Abdul Latif Khan
Gauhar Rehman
Sohn Eun-Young
Aamer Ali Shah
Kim Sang-Kuk
Joo Gil-Jae
Lee In-Jung
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Abstract
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Endophytic fungi are known for the production of valuable metabolites, but information on the gibberellin production capacity of this group is limited. We isolated 9 endophytic fungi from the roots of salt-stressed soybean plants and screened them on waito-c rice, in order to identify plant growth promoting fungal strains. The fungal isolate TK- 2-4 gave maximum plant length (20.35 cm) promotion in comparison with wild-type Gibberella fujikuroi (19.5 cm). In a separate experiment, bioassay of TK-2-4 promoted plant length and biomass of soybean cultivar Taegwangkong. The TK-2-4 culture filtrate was analyzed for the presence of gibberellins, and it was found that all physiologically active gibberellins, especially GA4 and GA7, were present in higher amounts (GA1, 0.11 ng/ml; GA3, 2.91 ng/ml; GA4, 3.21 ng/ml; and GA7, 1.4 ng/ml) in conjunction with physiologically inactive GA9 (0.05 ng/ml), GA12 (0.23 ng/ ml), GA15 (0.42 ng/ml), GA19 (0.53 ng/ml), and GA20 (0.06 ng/ml). The fungal isolate TK-2-4 was later identified as a new strain of Phoma herbarum, through the phylogenetic analysis of 28S rDNA sequence.
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KEYWORD
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Phoma herbarum, gibberellin production, endophytic fungi, soybean, growth promotion
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